奶牛胎盘脂联素、瘦素、内脂素与犊牛初生重相关性研究

doi:10.11843/j.issn.0366-6964.2017.01.022

摘要/Abstract

摘要：

旨在探究奶牛胎盘组织中脂联素（Adiponectin）、瘦素（Leptin）、内脂素(Visfatin)与犊牛初生重的相关性。选取规模化养殖场正常分娩奶牛56头，按犊牛初生重划分为A(≤40 kg)、B(40~45 kg)、C(≥ 45 kg)3组，分娩后立即手术采集胎盘组织，用 RT-PCR 和 ELISA 检测胎盘组织脂联素、瘦素和内脂素mRNA 和蛋白表达水平，分析其与犊牛初生重的相关性。结果表明，随着犊牛初生重增加，奶牛胎盘组织脂联素、内脂素mRNA 和蛋白表达水平均逐渐上升，瘦素先降低再升高；C组瘦素mRNA 表达水平显著高于B组（P<0.05），A组和B组、C组间差异均不显著（P>0.05），脂联素和内脂素在A、B、C 3组间差异不显著（P>0.05）；A、B组脂联素蛋白表达水平差异不显著（P>0.05），C组脂联素显著高于A组和B组（P<0.05），瘦素和内脂素在A、B、C 3组间表达水平差异不显著（P>0.05）；母牛胎盘脂联素、瘦素mRNA和蛋白表达水平均与犊牛初生重极显著正相关(P<0.01)，母牛胎盘内脂素mRNA 和蛋白表达水平均与犊牛初生重相关性不显著(P>0.05)；母牛胎盘脂联素、瘦素、内脂素mRNA 和蛋白表达水平两两之间均呈极显著正相关（P<0.01）。综上表明，奶牛胎盘中均有脂联素、瘦素和内脂素表达，胎盘脂联素、瘦素、内脂素相互协调对犊牛宫内生长发育发挥作用，其中脂联素和瘦素对犊牛初生重影响较大而内脂素影响较小。本研究结果为进一步研究奶牛胎盘组织中脂联素、瘦素、内脂素对犊牛初生重的作用机理提供参考。

Abstract:

In order to explore the correlation between adiponectin，leptin，visfatin in placenta and calf birth weight.56 naturally born healthy Chinese Holstein cows were used，in which，cows given birth to calf that were less than 40 kg were included in group A；between 40-45 kg were included in group B；more than 45 kg were included in group C.The placentas were surgically collected immediately after birth.RT-PCR and ELISA were used for evaluation of adiponectin，leptin，and visfatin in placenta，the correlation between adiponectin，leptin，visfatin in placenta and calf birth weight were analyzed.The results showed that，as the calf birth weight increased，the adiponectin and visfatin mRNA and protein expression levels increased，while leptin reduced first and then increased.The expression level of leptin mRNA of group C was significantly higher than that of group B (P<0.05). The difference among group A，B and C were not significant(P>0.05). Adiponectin and visfatin of group A，B and C were not significantly different (P>0.05). The expression level of adiponectin protein in group A and group B had no significant difference (P>0.05).The expression level of adiponectin in group C was significantly higher than that of group A and group B (P<0.05)，the expression levels of leptin and visfatin in group A，B and C had no significant differences(P>0.05). The mRNA and protein level of adiponectin and leptin in placenta were positively correlated with calf birth weight(P<0.01).The mRNA and protein level of visfatin in placenta with calf birth weight had no significant correlation(P>0.05).The mRNA and protein expression level of adiponectin，leptin and visfatin in 3 groups had no significant correlation respectively(P>0.05).The results demonstrated that adiponectin，leptin and visfatin were expressed in placenta of cows，which played a role in promoting of calf intrauterine growth cooperatively，adiponectin and leptin had great influence on calf birth weight，visfatin had little influence on calf birth weight.The study provided reference for studying mechanism between adiponectin，leptin，visfatin in placenta of cow and calf birth weight in the future.

中图分类号:

S852.2

沈留红，江涛，巫晓峰，姜思汛，肖劲邦，曹随忠，余树民，邓俊良，左之才，彭广能，马晓平，钟志军，任志华，王娅，胡延春. 奶牛胎盘脂联素、瘦素、内脂素与犊牛初生重相关性研究[J]. 畜牧兽医学报, 2017, 48(1): 185-192.

SHEN Liu-hong，JIANG Tao，WU Xiao-feng，JIANG Si-xun，XIAO Jin-bang，CAO Sui-zhong，YU Shu-min，DENG Jun-liang，ZUO Zhi-cai，PENG Guang-neng，MA Xiao-ping，ZHONG Zhi-jun，REN Zhi-hua，WANG Ya，HU Yan-chun. The Correlation between Adiponectin，Leptin，Visfatin in Placenta and Calf Birth Weight[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 185-192.

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